Quantitative Analysis of Oxygen Partial Pressure Measurement Based on Photoacoustic Pump-Probe Imaging

Pump-probe-based Photoacoustic imaging is an innovative technique for high-specificity molecular in deep tissues. In comparison to conventional photoacoustic imaging, this method effectively eliminates the interference from blood and other background signals, enabling detection of subtle target molecules. Additionally, by manipulating time delay between pump probe lasers, it facilitates non-invasive mapping oxygen partial pressure distribution within To quantify pump-probe we employed methylene blue as monitor changes a hemoglobin solution. Utilizing Gaussian noise model, investigated relationship stability triplet-state difference signal average number, also evaluated error associated with measuring pressure. The results demonstrated that under condition 200 averages, system achieved accuracy superior 33 mmHg range approximately 300 550 mmHg. This research will play significant role guiding further advancement application pump-probe-based technology.